Posts filed under ‘Bio-Plex Tutorial’
A few weeks ago we shared with you the first part in a two part series on 2D Gel Electrophoresis. In this last part of the series you will learn: IEF separation, using immobilized pH gradient (IPG) strips, running second-dimension PAGE, spot detection, gel matching, and data analysis, followed by Q&A on topics including sample preparation, storage, and running conditions.
The summer is almost coming to a close, but it is not too late for you summer students to still learn a thing or two! In this week’s intalment of the summer student tutorial series, we are proud to present you with another video courtesy of LabTricks on how to properly make and PH buffers for your experiment.
As always, if there are other tutorials that you are interested in, please let us know and we will do our best to post it in this space as soon as possible.
The video is courtesy of LabTricks which has no affiliation to Bio-Rad Laboratories.
For any quantitative immunoassay, the distinction between the dynamic range of an assay versus working range of an assay is an important consideration. Many believe the working range of an assay and the dynamic range of an assay are one and the same. However, this is not the case. Here we discuss the distinction between these two terms.
For quantitative immunoassays the dynamic range of an assay is described as the lowest to the highest concentration of an analyte that can be reliably detected by the assay. This is sometimes referred to as the lower and upper limits of detection (LLOD and ULOD, respectively). Although signal is detected, the accuracy and precision of this number may vary beyond what is acceptable to report as an accurate measure of the concentration of the target. Although still a useful measure, dynamic range is not as rigorous a measure of the true range of the assay.
For most labs, the working assay range is a more meaningful measure of the upper and lower limits of quantitation (ULOQ/LLOQ) of an assay. The working range of an assay is commonly defined as the range over which analyte concentrations can be quantitated with acceptable precision and reliability. Because this is a stricter measure and requires both sensitivity and accuracy, the working range is typically narrower than the dynamic range. However, it is a more reliable measure of the true range of concentrations that can be accurately quantitated.
As compared to the dynamic range, the values associated with working range of an assay are both precise (defined as how reproducible multiple measurements or calculations are) and accurate (defined as how close a measured or calculated quantity is to its true value).
Bio-Rad Laboratories is a leading provider of instrumention and assays for performing quantitative immunoassays. Bio-Rad’s Bio-Plex instrument is a powerful system for quantitative analysis of up to 100 different proteins, peptides, DNA fragments and RNA fragments from a single drop of sample.
In this latest video tutorial from Bio-Rad Field Application Specialist Dr. Sean Taylor, you will learn how to analyze your Bio-Plex experimental results using Bio-Plex Manager software.