Posts tagged ‘PCR’
Bruno Fonseca, PhD scientist at the Children’s Hospital of Eastern Ontario Research Institute and Riu Liu, PhD scientist at the University of Southampton (United Kingdom) collaborated over the past four years to develop a software tool that automates the design of primers for site-directed mutagenesis. Their new software program is called PrimerGenesis.
“We’ve automated a Nobel-prize winning research technique,” says Bruno Fonseca, co-creator of the software. “Genome sequencing yields vast amounts of data on disease-causing mutations in DNA. Investigating each mutation by a common practice called site-directed mutagenesis is a laborious and time-consuming task. There’s an appetite for automating this task in the field of molecular biology research, so it’s been rewarding to develop this software.”
Generating mutations in a gene typically requires a researcher to manually design a small strip of DNA called “primer”. Instead of counting individual nucleotides within a strip of DNA, this time-consuming task can be completed computationally in a matter of seconds by using this new software program.
“We’ve aimed to make it as user-friendly as possible,” said Rui Liu, co-creator of PrimerGenesis. “The software is extremely versatile and can be widely applied; yes, it can be used to generate mutations but it can also be used to introduce specific tags upstream or downstream of a gene.”
Thanks to CHEO for contributing this story.
To view the interactive tutorial visit http://www.biorad-ads.com/supermixes/content/
Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.
• Superior gene expression results — consistent performance in all cycling conditions
• Increased detection sensitivity and efficiency in compromised samples — Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors
• Decreased run times without compromising qPCR data quality
• Improved results with predeveloped qPCR assays — advanced formulation tolerates a broad range of reaction conditions, primer concentrations, and temperature ranges.
Bio-Rad has leveraged its 20 years of experience in PCR to develop an innovative, semi-automated heat sealing system for PCR microplates. The new PX1™ PCR plate sealer helps deliver a perfect seal by removing human variability from plate sealing. This newly introduced system helps eliminate a common problem in the thermal cycling workflow — sample evaporation. Evaporation during PCR can lead to the loss of precious samples and valuable research time. Additionally, sample evaporation may result in poor experimental uniformity by permitting larger standard deviations among replicate samples, complicating data interpretation. The PX1 PCR plate sealer delivers uniform temperature and pressure across a seal and plate, ensuring that every well is perfectly sealed and protected against sample evaporation.