A fantastic program for choosing the ideal reference genes

November 3, 2011 at 10:21 am 1 comment

Real time PCR for quantitative analysis of gene expression has become an integral part of many (if not most) research labs around the world. When preparing for qPCR analysis, it is important to choose reference genes than do not vary under experimental conditions and that can be used to normalize your data to accurately reflect changes due to the given treatment. (See: Are you using the right reference genes?)

Choosing the right reference genes can be a very difficult task. Most experts agree that when performing qPCR for gene expression, the more reference genes used, the merrier. Unfortunately, “the more the merrier” is also associated with “the more, the more complicated.”

Fortunately, Dr. Sean Talyor, Field Application Specialist at Bio-Rad Canada has found a great program that makes use of many online databases to help you find the ideal reference genes. In the following tutorial, Sean will show you how to use the Refgenes program for choosing your ideal reference genes. The program utilizes a massive microarray database to help determine gene expression under a variety of experimental conditions.

Entry filed under: Bio-Rad Tutorial, PCR Tutorial, PCR Video Tutorials, Tools. Tags: , , .

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1 Comment Add your own

  • […] 0 comments This gem was originally posted in August 2010, however, in consideration of our post on reference gene selection posted earlier this week, I thought that now would be a good time to share this information once […]

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