More droplet digital PCR educational resources

December 1, 2011 at 4:17 am Leave a comment

Like any PCR “gear-head,” I am always excited to learn about new amplification technologies and that is why I find myself once again discussing  Bio-Rad’s acquisition of QuantaLife , the droplet digital PCR company.

Droplet Digital PCR  uses microfluidics to partition each sample into 20,000 to millions of one-nanoliter droplets, each of which contains a separate PCR. The droplets are streamed in single file past a detector to count the negative and positive reactions.

The QuantaLife system introduces a new era of high performance PCR testing. The system detects slight differences in gene copy number (i.e., 6 copies from 5), or a 10% difference in mRNA expression. There is no need for a standard. The system can read 48 samples per hour and includes a droplet generator and a droplet reader, quantifies DNA molecules using the company’s patented high-resolution, Droplet Digital PCR technology – a 3rd generation PCR technology that enables single-molecule resolution of target sequences.

A ton of information on digital PCR can be found over at the Gene Quantification  site and we are in the process of producing a video tutorial on droplet digital PCR which will be given by our beloved Field Application Specialist Dr. Sean Taylor. Please check back on this site either later today or tomorrow to watch this exciting and educational video!

Entry filed under: Droplet Digital PCR, PCR. Tags: , , , .

Big Bucks for BC Video Introduction to Droplet Digital PCR

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