Which camera is best for chemiluminescent detection?

January 30, 2012 at 8:33 am Leave a comment

As illustrated in the chart below, there are numerous methods for documenting your western blotting results. In this post we will review the use of charged couple display (CCD) cameras for chemiluminescence detection of western blots. The information is reprinted from the Protein Blotting Guide from Bio-Rad Laboratories. Click on the picture to enlarge the chart.

Charged couple display (CCD) imaging is the easiest, most accurate, and rapid method for chemiluminescent detection. CCD cameras are versatile systems that image both gels and blots, and operate with either trans-illumination provided by light boxes (visible or UV) positioned underneath the gel for imaging a variety of stains (Coomassie, silver, fluorescence) or epi-illumination of blots detected using colorimetric or fluorescence techniques. Different illumination wavelengths are available for multiplex fluorescence immunodetection. CCD cameras can also be used without illumination to detect luminescent signals. Supercooled CCD cameras reduce image noise, allowing detection of faint luminescent signals.

CCD cameras have a linear response over a broad dynamic range — 2–5 orders of magnitude — depending on the bit depth of the system. CCD cameras also offer convenience by providing a digital record of experiments for data analysis, sharing, and archiving, and by eliminating the need to continually purchase consumables for film development. CCD cameras also approach the limit of signal detection in a relatively short time. For example, the VersaDoc™ MP 5000 imaging system can reach the limit of detection of a given experiment in <1 min, compared to 30 min required by Kodak Bio-Max film for the same experiment.

For more information on Bio-Rad’s line of imaging systems visit www.bio-rad.com/imaging.

Entry filed under: Bio-Rad Tech Note, protein blotting, Proteomic Technotes, Western Blot and ELISA. Tags: , , .

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