Top 5 factors affecting protein migration

February 20, 2012 at 3:40 pm Leave a comment

Determining the molecular weight (MW) of proteins is a basic goal of many studies. Although the gold standard for determining MW is mass spectrometry, this technique involves costly and time consuming steps. As such, SDS-PAGE using molecular weight protein standards has become an excellent substitute to mass spectrometry.

Despite the fact we often use protein standards for MW determination, many of us are unfamiliar with just how many factors influence protein migration and the usefulness of protein standards.

For example, can you name the top 5 factors that influence protein migration?

Did you know that despite being labelled as having the exact same MW, standards from different manufacturers will often have different gel migration patterns?

How can we deal with these inconsistencies and what else do we need to know in order to make sure that our results are as accurate as possible?

For answers to these and other questions, check out the attached article from Bio-Rad Laboratories.

Entry filed under: Bio-Plex Technotes, protein blotting, Proteomic Technotes, Proteomics, Western Blot and ELISA. Tags: , , .

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