Improve your confidence in western blotting results

March 26, 2012 at 1:55 pm Leave a comment

Do you use housekeeping proteins to normalize your western blotting results? If not, you probably should! Normalizing with internal controls helps minimize quantitative errors that can result from inconsistent sample preparation and sample load variability. Nonetheless, normalization with housekeeping proteins has its challenges. Such challenges include:

  • choosing a housekeeping protein that definitely does not change expression under the specific conditions being studied
  • loss of signal due to stripping and reprobing of membrane
  • uneven loss of protein from membrane during stripping process which can compromise results

Fortunately, multiplex fluorescent western blotting and the use of stain-free technologies can help overcome some of the challenges described above. Check out Stain-Free Approach for Western Blotting: Alternative to the Standard Blot Normalization Process published in Genetic Engineering & Biotechnology News to learn more about how these technologies can help you improve your confidence in your western blotting results.

Entry filed under: Bio-Rad Tech Note. Tags: , , .

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