Factors Impacting Gene Expression Analysis

May 23, 2012 at 2:54 pm Leave a comment

There are many factors that impact gene expression analysis. They include:

RNA Isolation

  • RNA integrity, purity and yield
  • Genomic DNA contamination
  • Inhibitors of cDNA synthesis and qPCR
  • RNase and DNase contamination

Reagents-Reverse Transcritption

  • cDNA synthesis efficiency
  • RNA protection
  • Input RNA capacity
  • Accurate representation of mRNA

Reagents-Real-Time qPCR

  • Detection sensitivity
  • Assay specificity
  • Inhibitors in sample
  • Reproducibility of thermal cycling conditions and instrumental compatibility

PCR Plastic Consumables

  • Instrument compatibility
  • Optimum performance
  • Automation friendly
  • Potential source of contamination and inhibition

Fortunately, Bio-Rad Laboratories has solutions to help you overcome these issues. Such as their huge selection of reverse transcription tools (click to enlarge):

For more great information, download the Amplification Brochure from Bio-Rad Laboratories.

Entry filed under: Bio-Rad Product Highlight. Tags: , .

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