Bored to death by western blotting

May 29, 2012 at 2:16 pm Leave a comment

One of my earliest experiences in the lab was as a summer student performing tons and tons of western blots. The technique seemed so repetitive and boring. Pour gel, prepare samples, load gel, run gel, transfer proteins, block, incubate, wash, wash, wash, wash, incubate, wash, wash, wash, develop, expose to film and…….BAD RESULTS! REPEAT, REPEAT, REPEAT!!!!! All my friends were finished their jobs working in a local day camp and here I was washing a PVDF membrane and praying to the gods of x-ray development. I was bored out of my brain and I felt like I was wasting my time and my talents. Does this resonate with anybody? There had to be a better way.

Thankfully, Bio-Rad Laboratories has now released a novel approach to Western Blotting. The V3 Western Workflow cuts Western blotting time in half and significantly improves results. Don’t believe me? Checkout the gels below. See the difference?

Care to read about a real life example? Checkout the article Novel V3 Western Workflow Revolutionizes COPD Protein Research.

Entry filed under: Western Blot and ELISA. Tags: , .

How to bring a frog back from the dead Hats off to everyone’s favourite gel

Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )


Connecting to %s

Trackback this post  |  Subscribe to the comments via RSS Feed


Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

%d bloggers like this: