Bored to death by western blotting

May 29, 2012 at 2:16 pm Leave a comment

One of my earliest experiences in the lab was as a summer student performing tons and tons of western blots. The technique seemed so repetitive and boring. Pour gel, prepare samples, load gel, run gel, transfer proteins, block, incubate, wash, wash, wash, wash, incubate, wash, wash, wash, develop, expose to film and…….BAD RESULTS! REPEAT, REPEAT, REPEAT!!!!! All my friends were finished their jobs working in a local day camp and here I was washing a PVDF membrane and praying to the gods of x-ray development. I was bored out of my brain and I felt like I was wasting my time and my talents. Does this resonate with anybody? There had to be a better way.

Thankfully, Bio-Rad Laboratories has now released a novel approach to Western Blotting. The V3 Western Workflow cuts Western blotting time in half and significantly improves results. Don’t believe me? Checkout the gels below. See the difference?

Care to read about a real life example? Checkout the article Novel V3 Western Workflow Revolutionizes COPD Protein Research.

Entry filed under: Western Blot and ELISA. Tags: , .

How to bring a frog back from the dead Hats off to everyone’s favourite gel

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