Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip
Surface Plasmon Resonance (SPR) is an optical phenomenon that is used for the label-free analysis of the binding of any two molecules in real time. An SPR biosensor instrument generates a sensorgram by monitoring the change in the SPR response with time. Fitting is possible with an instrument-generated sensorgram, and fitting the sensorgram to a suitable kinetic model allows for the calculation of kinetic parameters such as the association (ka) and dissociation (kd) rate constants. SPR is an important tool for characterizing biomolecular interactions.