Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip

December 27, 2012 at 3:59 pm Leave a comment

Surface Plasmon Resonance (SPR) is an optical phenomenon that is used for the label-free analysis of the binding of any two molecules in real time. An SPR biosensor instrument generates a sensorgram by monitoring the change in the SPR response with time. Fitting is possible with an instrument-generated sensorgram, and fitting the sensorgram to a suitable kinetic model allows for the calculation of kinetic parameters such as the association (ka) and dissociation (kd) rate constants. SPR is an important tool for characterizing biomolecular interactions.

Read more…

Entry filed under: Bio-Rad Tech Note. Tags: , .

Top biotechnology news story in 2012 Top 50 Industry Voices in Biotech Twittersphere — 2012

Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )


Connecting to %s

Trackback this post  |  Subscribe to the comments via RSS Feed


Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

%d bloggers like this: