Analyzing Binding Kinetics with Surface Plasmon Resonance Complemented with Direct Mass Spectrometry on the Same Sensor Chip

December 27, 2012 at 3:59 pm Leave a comment

Surface Plasmon Resonance (SPR) is an optical phenomenon that is used for the label-free analysis of the binding of any two molecules in real time. An SPR biosensor instrument generates a sensorgram by monitoring the change in the SPR response with time. Fitting is possible with an instrument-generated sensorgram, and fitting the sensorgram to a suitable kinetic model allows for the calculation of kinetic parameters such as the association (ka) and dissociation (kd) rate constants. SPR is an important tool for characterizing biomolecular interactions.

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