Free Article: Stain-Free Approach to Western Blotting
Do you think that Western Blotting, the “vanilla” technique for all molecular biologists, has reached its peak? Are you skeptical that there is any room to futher improve upon the way western blots are typically done? Do you believe that seeing is believing when it comes to westerns? If you answered yes to any of these questions, you better thinkk again.
In an article highlighting alternatives to the standard blot normalization process Ryan Short and Anton Posch of Bio-Rad Laboratories explore the strengths and pitfalls of standard housekeeping protein normalization techniques and suggest an innovative technology for improving upon what was once thought of as the most mature technology in the molecular biologist’s arsenal.
The article analyzes the following protocols:
- strip and re-probe
- multiplex fluorescent western blotting
- protein profiling studies
- stain free technology
For more information, download the free report Stain-Free Approach for Western Blotting.