Immobilization of Active Kinases for Small Molecule Inhibition Studies
Surface plasmon resonance (SPR) is a well-established and important screening tool in the small molecule drug discovery workflow. Using the Bio-Rad ProteOn™ XPR36 protein interaction array system, an SPR biosensor platform, rapid optimization of immobilization conditions for a kinase target was carried out to ensure that the immobilized protein remains in an active state for the following screening step. Two kinase inhibitor compounds were screened against the kinase target to identify positive hits and to obtain binding kinetics. The whole experiment was completed in a fast workflow with a single sensor chip, showing the high performance of the ProteOn XPR36 system.