A Defined Methodology for Reliable Quantification of Western Blot Data

June 12, 2013 at 3:28 pm Leave a comment

gelChemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. This is mainly due to the various steps, techniques, reagents, and detection methods that are used to obtain the associated data. In order to have confidence in densitometric data from western blots, researchers should be able to demonstrate statistically significant fold differences in protein expression. This entails a necessary evolution of the procedures, controls, and the analysis methods. We describe a methodology to obtain reliable quantitative data from chemiluminescent western blots using standardization procedures coupled with the updated reagents and detection methods.

Read: A Defined Methodology for Reliable Quantification of Western Blot Data

Entry filed under: Bio-Rad Tech Note. Tags: , , .

Musical: Bright Scope-Long Lab Coat Video Tutorial: Perfect Your Quantitative Western Blotting

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