Using Bio-Rad’s PDQuest™ Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting

August 7, 2013 at 2:04 pm Leave a comment

Two-dimensional (2-D) electrophoresis is a widely used gel-based method for separating protein mixtures by charge (isoelectric point) and size (molecular weight). After 2-D electrophoresis (2-DE; described in detail in the Bio-Rad Applications & Technologies section), gels can be processed for protein detection via total protein staining and/or western blotting. Western blotting analysis following 2-DE separation is particularly useful in at least two important applications: (1) identification of pathogen-derived proteins that are highly immunogenic (have potential as vaccine candidates), and (2) evaluation of the range of host cell protein (HCP) impurities detectable by polyclonal antibodies used to monitor HCP impurities in purified biopharmaceuticals. For both applications, it is critical to generate a match rate from an overlay of the 2-DE and western blotting analyses. Bio-Rad’s PDQuest 2-D analysis software can be used to easily generate match rates. We describe here, step-by-step guidelines for generating this information using PDQuest software.

Read more…

Entry filed under: Bio-Rad Tutorial. Tags: , , .

Canada’s Life Sciences Clusters Ever heard of synesthesia? Check out this story!

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Trackback this post  |  Subscribe to the comments via RSS Feed


biorad1

Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact info@cbt20.ca for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

Error: Twitter did not respond. Please wait a few minutes and refresh this page.


%d bloggers like this: