The Answer to Fading Western Blots
Publications have become very strict when it comes to publishing research that includes images of blots, often requiring images of the full blot, uncut. To keep the membranes uncut, researchers often reprobe which can cause the marker to fade.
Bio-Rad has launched the brightest protein marker available to researchers which addresses such challenges. The Precision Plus Dual Protein Standard is not only brighter, but also stays on the membrane longer. This allows researchers to identify proteins more effectively and have greater confidence in their western blots.
The strong marker persistence of the Precision Plus Protein™ Dual Color Standards throughout electrophoresis and western blotting, even during rigorous stripping and reprobing protocols, makes the product a more effective tool for monitoring separation and estimating protein molecular weights.
“Providing the images of the entire blot is good practice for science and publications,” said Dr. Federica del Monte, assistant professor of medicine at Harvard Medical School. “In order to reprobe for the loading control and keep the membranes uncut, we sometimes need to strip them. This often results in fading of the markers. The ability to publish clear blots with the marker still on is very important.”
Key benefits of the new Precision Plus Protein Dual Color Standards include:
- Increased brightness — better identification of target proteins on gels and blots as well as easier monitoring of gel electrophoresis and confirmation of transfer quality
- Stronger marker persistence — marker remains on the blot even under the most rigorous conditions, boosting confidence in your western blots; stronger marker persistence also facilitates accurate molecular weight estimation
Bands in the Precision Plus Protein Dual Color Standards range from 10 to 250 kD with sharp pink reference bands at 25 and 75 kD for simple visualization. The new Dual Color Standards share the same migration pattern as their previous iteration.