The Key to Consistent Crystal Generation

July 8, 2014 at 3:46 pm Leave a comment

PPARg plays a role in glucose metabolism. Activated PPARg increases insulin sensitivity, making PPARg an important receptor for treatment of type 2 diabetes. Obtaining a crystal structure of PPARg aids in development of interacting ligands and beneficial anti-diabetic drugs. Here we describe the purification of PPARg using Bio-Rad’s NGCTM chromatography system and a three-step column purification approach. Well-ordered crystals of the purified PPARg protein were obtained. X-ray diffraction data showed consistent results compared to established protocols.

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Entry filed under: Bio-Rad Tech Note. Tags: , .

Not the Scientist I Used To Be Eight Days a Week (in the lab)

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