The Key to Consistent Crystal Generation
PPARg plays a role in glucose metabolism. Activated PPARg increases insulin sensitivity, making PPARg an important receptor for treatment of type 2 diabetes. Obtaining a crystal structure of PPARg aids in development of interacting ligands and beneficial anti-diabetic drugs. Here we describe the purification of PPARg using Bio-Rad’s NGCTM chromatography system and a three-step column purification approach. Well-ordered crystals of the purified PPARg protein were obtained. X-ray diffraction data showed consistent results compared to established protocols.