Everything You Need to Know About Droplet Digital PCR

November 25, 2014 at 3:23 pm Leave a comment

ddpcr guideDroplet Digital polymerase chain reaction (ddPCR™) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions. Droplet Digital PCR using Bio-Rad’s QX100™ or QX200™ Droplet Digital PCR system overcomes the previous lack of scalable and practical technologies for digital PCR implementation.

ddPCR has the following benefits for nucleic acid quantification:

  • Unparalleled precision — the massive sample partitioning afforded by ddPCR enables small fold differences in target DNA sequence between samples to be reliably measured
  • Increased signal-to-noise — enrich for rare targets by reducing competition that comes from high-copy templates
  • Removal of PCR efficiency bias — error rates are reduced by removing the amplification efficiency reliance of PCR, enabling accurate quantification of targets
  • Simplified quantification — a standard curve is not required for absolute quantification

Download the Droplet Digital™ PCR Applications Guide for a comprehensive explanation of this powerful technique.

The guide includes chapters on:

  1. Designing Droplet Digital™ PCR Experiments
  2. Absolute Quantification and the Statistics of Droplet Digital™ PCR
  3. Copy Number Variation Analysis
  4. Rare Mutation and Sequence Detection
  5. Gene Expression
  6. Next-Generation Sequencing Library Analysis
  7. Droplet Digital™ PCR Tips, Assay Considerations, and Troubleshooting

Entry filed under: Bio-Rad Tutorial, Droplet Digital PCR. Tags: , .

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