Everything You Need to Know About Droplet Digital PCR

November 25, 2014 at 3:23 pm Leave a comment

ddpcr guideDroplet Digital polymerase chain reaction (ddPCR™) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions. Droplet Digital PCR using Bio-Rad’s QX100™ or QX200™ Droplet Digital PCR system overcomes the previous lack of scalable and practical technologies for digital PCR implementation.

ddPCR has the following benefits for nucleic acid quantification:

  • Unparalleled precision — the massive sample partitioning afforded by ddPCR enables small fold differences in target DNA sequence between samples to be reliably measured
  • Increased signal-to-noise — enrich for rare targets by reducing competition that comes from high-copy templates
  • Removal of PCR efficiency bias — error rates are reduced by removing the amplification efficiency reliance of PCR, enabling accurate quantification of targets
  • Simplified quantification — a standard curve is not required for absolute quantification

Download the Droplet Digital™ PCR Applications Guide for a comprehensive explanation of this powerful technique.

The guide includes chapters on:

  1. Designing Droplet Digital™ PCR Experiments
  2. Absolute Quantification and the Statistics of Droplet Digital™ PCR
  3. Copy Number Variation Analysis
  4. Rare Mutation and Sequence Detection
  5. Gene Expression
  6. Next-Generation Sequencing Library Analysis
  7. Droplet Digital™ PCR Tips, Assay Considerations, and Troubleshooting

Entry filed under: Bio-Rad Tutorial, Droplet Digital PCR. Tags: , .

I Want a Clone How I Became a Leech Expert

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )


Connecting to %s

Trackback this post  |  Subscribe to the comments via RSS Feed


Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact info@cbt20.ca for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

%d bloggers like this: