Everything You Need to Know About Droplet Digital PCR
Droplet Digital polymerase chain reaction (ddPCR™) was developed to provide high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions. Droplet Digital PCR using Bio-Rad’s QX100™ or QX200™ Droplet Digital PCR system overcomes the previous lack of scalable and practical technologies for digital PCR implementation.
ddPCR has the following benefits for nucleic acid quantification:
- Unparalleled precision — the massive sample partitioning afforded by ddPCR enables small fold differences in target DNA sequence between samples to be reliably measured
- Increased signal-to-noise — enrich for rare targets by reducing competition that comes from high-copy templates
- Removal of PCR efficiency bias — error rates are reduced by removing the amplification efficiency reliance of PCR, enabling accurate quantification of targets
- Simplified quantification — a standard curve is not required for absolute quantification
Download the Droplet Digital™ PCR Applications Guide for a comprehensive explanation of this powerful technique.
The guide includes chapters on:
- Designing Droplet Digital™ PCR Experiments
- Absolute Quantification and the Statistics of Droplet Digital™ PCR
- Copy Number Variation Analysis
- Rare Mutation and Sequence Detection
- Gene Expression
- Next-Generation Sequencing Library Analysis
- Droplet Digital™ PCR Tips, Assay Considerations, and Troubleshooting