Bio-Rad Introduces the ddPCR™ KRAS Screening Multiplex Kit for Cancer Research

January 21, 2015 at 4:12 pm Leave a comment

Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) today announced the launch of the ddPCR™ KRAS Screening Multiplex Kit, the latest offering in the company’s Droplet Digital™ PCR line of predesigned, fully wet-lab validated assays. The ddPCR KRAS Screening Multiplex Kit offers improved sensitivity and simultaneous detection of the seven most common KRAS mutations in a single ddPCR experiment.

KRAS mutations are found in 90% of pancreatic cancer cases and 30% of colorectal cancer cases, making it a gene of great interest to cancer researchers. The Multiplex Kit is able to detect and quantify extremely low levels (down to 0.2%) of KRAS mutant DNA in a single assay. Cancer samples are often available in limited quantities. Using the Multiplex Kit, researchers can derive results from low amounts of starting material.

“The KRAS Screening Multiplex Kit delivers a simple yes/no answer as to whether a KRAS mutation exists in smaller samples,” said Dr. Filip Janku of the University of Texas MD Anderson Cancer Center in Houston, TX. “If researchers were to test for the presence of each possible mutation they could end up performing seven to ten separate PCR experiments only to discover the sample was negative. Multiplex kits such as the KRAS Screening Multiplex Kit allow researchers to screen for multiple mutations in a single reaction, saving time and costly resources.”

Bio-Rad’s ddPCR technology provides an absolute measure of target DNA molecules without the need for a standard curve. Together with the ddPCR KRAS Screening Multiplex Kit, this technology enables detection of a single mutant molecule in a background of 2,000 wild-type molecules (0.05% mutation frequency). Measuring these extremely low levels of mutation abundance could lead to the development of new, less invasive and more sensitive cancer diagnostics.

ddPCR KRAS Screening Multiplex Kits are compatible with all of Bio-Rad’s Droplet Digital PCR Systems, including the QX200™ AutoDG™ Droplet Digital™ PCR System.

For more information on Bio-Rad’s ddPCR KRAS Screening Multiplex Kit, please visit

Entry filed under: Bio-Rad Product Highlight.

Pipetting to the Oldies

Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

Trackback this post  |  Subscribe to the comments via RSS Feed


Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

%d bloggers like this: