Investigating Cancer Stem Cells

Learn Total Protein Normalization from the Experts

Image Lab™ Software-Learn Total Protein Normalization from the Experts

Join us for a 30 minute live webinar developed and delivered by our knowledgeable Technical Support Team.

Tuesday, December 9, 2014 | 10:00 AM Pacific

As you get ready to use your new system, we will provide you with an opportunity to learn about total protein normalization using Stain-Free Technology. This training will cover the steps to use Image Lab software to normalize your western blot data, as well as provide guidelines for using housekeeping proteins as loading controls.

Walk-Away Automation for Accurate and Reliable Cell Sorts

The S3e Cell Sorter’s simple setup and walk-away automation allow researchers to sort cells in their own labs.

Bio-Rad Laboratories announced the launch of its S3e Cell Sorter that offers walk-away automation for accurate and reliable cell sorts of one or two populations.

The S3e Cell Sorter builds on the original S3™ Cell Sorter technology with the addition of the new AutoGimbal System. This novel automated system perfectly aligns the nozzle tip and stream to the optics, eliminating the need for manual repositioning. The AutoGimbal System makes these fine adjustments using five motorized controllers. Researchers can now be more confident that their system is set up correctly every time.

Traditionally, researchers have sorted cells at a core lab due to high instrument and operating costs, as well as the need for a trained core staff specialist to help run the sort. The arrival of the affordable and first walk-away S3e Cell Sorter enables labs to have their own personal cell sorter. The simple setup and hands-free calibration eliminate the pain points of typical high-end instruments, making cell sorting easier and accessible to researchers who are increasingly using these instruments for low-complexity sorts.

Additional benefits of the S3e Cell Sorter include:

• Simplified setup — fully automated drop delay calculation and droplet break-off monitoring enable precise sorting with minimal training
• Small benchtop footprint — onboard fluidics and temperature control allow a compact size (2.3 x 2.1 x 2.1 ft)
• Two-way cell sorting — sort two different defined populations at the same time with one or two lasers and up to four fluorescent detectors
• High speed and high purity — sort cells quickly while maintaining high sensitivity and purity

For more information, please visit www.bio-rad/S3ePR for the S3e and other available Bio-Rad cell sorters.

Cell Sorter Reviews

Earlier this week,  The Scientist reviewed and compared five different cell sorters. The comparison included price, size, number of lasers, colour detection and sample format. Bio-Rad Laboratories S3 Cell Sorter was the first to be reviewed and was given accolades for its ease of use, user friendly software and the novel internal fluidics system. The reviewer interviewed professor Stacy Blaine from SUNY Downstate Medical who  said that the system, which requires very little training, enabled her to leave others to use the sorter unsupervised. This gave her the freedom to do other work in the time that it would normally take to train new users on the system.

To learn more about cell sorting options visit Sorting Made Simpler: A guide to affordable, compact fluorescence-activated cell sorters

Watch the video below to see how some of the design innovations in the S3 Cell Sorter came about, including a unique automated method for calculating drop delay.

A Fast Approach to Staining Nuclei