Posts tagged ‘BioPlex’

Identifying Cardiac Defect-Related Protein Signatures

Dr Francis Spinale. Professor of Cardiothoracic Surgery; Medical University of South Carolina

“The first opportunity I had to lay my hand on a human heart I knew that was that — I knew that I wanted to know how the heart works, but more importantly, how it doesn’t work,” says Dr Francis Spinale, describing what inspired him when he was a PhD candidate to become not only an important researcher in the field of cardiac disease, but also a clinical practitioner to help treat patients with disease. This experience led the doctoral student to combine his PhD studies with those toward a medical degree, and it was during his first year in medical school that this initial inspiration turned to true passion.

“I did a rotation in pediatric cardiology, and that’s the first time I encountered children with life-threatening congenital heart defects,” says Spinale. “One evening, I was the unfortunate first-year student to watch an infant die of congenital heart disease. So there was the magic and amazement of watching the human heart work, and then there was the tragedy and unfairness of the fact that young lives are lost to heart disease before they even get the chance to do anything wrong.” So with these two juxtaposed influences, Spinale completed medical school with the purpose of gaining clinical knowledge and experience to go back to the research laboratory fully armed to understand the biological basis of cardiac disease.

Click here to read more.

September 15, 2011 at 9:34 am Leave a comment

Programming Bio-Plex Manager software

A step-by-step tutorial on how to program Bio-Rad Laboratories’ Bio-Plex Manager software. Given by Dr. Sean Taylor, Field Applications Specialist, Bio-Rad Laboratories.

April 14, 2011 at 8:20 am Leave a comment

Preparing samples for Bio-Plex analysis

Dr. Sean Taylor is a field applications specialist for Bio-Rad Laboratories and spends most of his days training scientists on how to acheive their research goals or improve upon their proteomic or genomic techniques.

In the following two videos, Sean shows Bio-Plex customers how to prepare their samples for Bio-Plex analysis.

March 28, 2011 at 7:18 am 1 comment

Multiplex Assays for Human Diabetes Biomakers

Profiling of multiple diabetes and metabolic biomarkers allows researchers to better understand the complex interactions among adipokines, gut hormones, and other biomolecules associated with diabetes and metabolic dysfunctions.

The attached paper, Development and Validation of Multiplex Assays for Human Diabetes Biomarkers describes a multiplex immunoassay for simultaneous quantitaton of diabetes and metabolic biomarkers in human serum and plasma of up to 12 targets, including adiponectin, adipsin, C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, resistin and visfatin.

The targets can also be multiplexed with other cytokine biomarkers which have been shown to play an important role in metabolic research and immune response. For more information on assays that allow for multiplexing cytokine and diabetes biomarkers see our previous post multiplexing cytokine and diabetes biomarkers.

September 6, 2010 at 5:19 am Leave a comment

How to Increase the Efficiency of Your Cytokine BioMarker Study

Many articles have been written on the importance of cytokines and chemokines in immune response and their utility as biomarkers. Some of the most recent publications include:

A recent review in Toxicological Sciences where J. Tarrant discusses the role that cytokines, chemokines, and growth factors play in inflammation, immune response, and repair and their applicability in biomarker studies.

Tarrant, J. (2010). Blood Cytokines as Biomarkers of in vivo Toxicity in Preclinical Safety Assessment: Considerations for Their Use Toxicological Sciences DOI: 10.1093/toxsci/kfq134

Lumachi’s et al. review on cytokines, thyroid diseases and thyroid cancer published in Cytokine which discusses the utility of cytokines as serum biomarkers of thyroid disease.

Lumachi, F., Basso, S., & Orlando, R. (2010). Cytokines, thyroid diseases and thyroid cancer Cytokine, 50 (3), 229-233 DOI: 10.1016/j.cyto.2010.03.005

And a thorough review of the role of various cytokines as biomarkers relating to oxidative stress and inflammation in Alzheimer’s disease appeared in the February edition of Biomarkers in Medicine.

Galasko, D., & Montine, T. (2010). Biomarkers of oxidative damage and inflammation in Alzheimer’s disease Biomarkers in Medicine, 4 (1), 27-36 DOI: 10.2217/bmm.09.89

Bio-Rad’s BioPlex multiplex bead array system is one of the most common tools for identifying and quantifying cytokine biomarkers in a multiplex environment. The Bio-Plex Pro human and mouse Group I and Group II panels have a magnetic bead backbone which simplifies the assay process by enabling automatic washes in a magnetic washing station. The effectiveness of the immunoassays depend on their analytical sensitivity and low cross-reactivity between cytokine antibodies. The attached bulletin shows that the majority of Bio-Rad’s Bio-Plex Pro group I and II cytokines may be combined in a 48-plex assay for the detection and quantification of human cytokines and in a 32-plex assay for the detection and quantification of mouse cytokines making the Bio-Plex Pro cytokine panel an extremely efficient tool for biomarker research.

Multiplexing Across Cytokine Panels

May 17, 2010 at 5:40 am Leave a comment


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