Posts tagged ‘Diabetes’

One step closer to cloning humans

Researchers said Wednesday they used a cloning technique to create human embryos that were close genetic copies of the people from which they were derived—a potentially significant breakthrough in the quest to develop patient-specific stem cells to treat serious diseases.

Scientists involved in the experiment, which was published in the journal Nature, created 13 early-stage human embryos that were partial genetic clones of diabetic patients. The copies were not identical, as each embryo carried three sets of chromosomes—an extra set. That means they were abnormal and wouldn’t have been viable if implanted in a womb and carried to term.

Click to read more from the Wall Street Journal.

October 6, 2011 at 4:23 pm 1 comment

Treating obesity in the gut-brain axis

Suraj Unniappan, associate professor in York’s Department of Biology, Faculty of Science & Engineering, is delving into the metabolic effects of a protein called nesfatin-1, abundantly present in the brain. His studies found that rats administered with nesfatin-1 ate less, used more stored fat and became more active. In addition, the protein stimulated insulin secretion from the pancreatic beta cells of both rats and mice.

“[The rats] actually ate more frequently but in lesser amounts,” says Unniappan, a member of York’s neuroscience graduate diploma program, and a recipient of a Canadian Institutes of Health Research (CIHR) New Investigator Award. “In addition, they were more active and we found that their fatty acid oxidization was increased. In other words, the energy reserve being preferably used during nesfatin-1 treatment was fat. This suggests more fat loss, which could eventually result in body weight loss,” he says.

The findings were reported in two recent research articles from Unniappan’s laboratory: one published today in Endocrinology and another in March 2011 in Journal of Endocrinology.

To read more click here.

August 10, 2011 at 10:54 am Leave a comment

Multiplex Assays for Human Diabetes Biomakers

Profiling of multiple diabetes and metabolic biomarkers allows researchers to better understand the complex interactions among adipokines, gut hormones, and other biomolecules associated with diabetes and metabolic dysfunctions.

The attached paper, Development and Validation of Multiplex Assays for Human Diabetes Biomarkers describes a multiplex immunoassay for simultaneous quantitaton of diabetes and metabolic biomarkers in human serum and plasma of up to 12 targets, including adiponectin, adipsin, C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, resistin and visfatin.

The targets can also be multiplexed with other cytokine biomarkers which have been shown to play an important role in metabolic research and immune response. For more information on assays that allow for multiplexing cytokine and diabetes biomarkers see our previous post multiplexing cytokine and diabetes biomarkers.

September 6, 2010 at 5:19 am Leave a comment

Petri Dish Pancreas

Dr. Ian Rogers of Mount Sinai Hospital in Toronto is working on a replacement pancreas that would be grown in a lab and then placed in those with Type 1 diabetes to restore their insulin production.

Rogers’s team is building a pancreas out of a surgical sponge, a three-dimensional structure seeded with insulin-producing islet cells. The pancreas would be grown in the lab and then placed under the skin of those with Type 1 diabetes to restore their insulin production.

Read more from CBC regrowing body parts closer to reality

In salute of Dr. Rogers and those involved in diabetes research, we present you with “Weird Al” Yankovic’s tribute to the pancreas.

August 9, 2010 at 4:42 am Leave a comment

Multiplexing Diabetes and Cytokine BioMarkers

Bio-Rad Laboratories recently announced the launch of 2 multiplex bead array panels for scientist engaged in Diabetes research. The assays are for the detection of 8 mouse and 10 human biomarkers of diabetes and obesity and can be run on Bio-Rad’s Bio-Plex instrument (or other Luminex based platforms).

The Bio-Plex Pro Diabetes Assays only require 12.5 ug of sample and will produce accurate and sensitive results in under 3 hours.

In recognition of the important role that cytokines play in the progression of diabetes, Bio-Rad has ensured that the Bio-Plex Pro Diabetes Assay is fully compatible with its Bio-Plex Pro cytokine, chemokine and growth factor assays. Now diabetes researchers can perform a multi-plex analysis in order to quantify the most important diabetes biomarkers AND cytokine, chemokine and growth factor expression from just 12.5 ug of sample…all in the same tube! This is a huge benefit to any diabetes researcher as it helps eliminate inter-experimental variability that can arise when quantifying analytes from different aliquots of the same sample.

The Bio-Plex Pro Mouse Diabetes Assay includes a multiplex analysis of Ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 and Resistin. The Bio-Plex Pro Human Diabetes Assay quantifies levels of c-peptide, visfatin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 and Resistin in your 12.5 ul sample.

In the attached tech note: Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays: Human and Mouse Panels, Zimmerman et al. demonstrate the compatibility of 40 human cytokine assays with all 10 human diabetes assays and 32 mouse cytokine assays with all 8 mouse diabetes assays. The data includes an analysis of sensitivity, specificity and accuracy with limits of detection as low as 0.1pg/ml for several cytokine and diabetes biomarkers.

Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays: Human and Mouse Panels

July 5, 2010 at 8:48 am 1 comment

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