Posts tagged ‘electrophoresis’
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FastCast acrylamide solutions bring the latest chemistry in gel electrophoresis to those who hand cast gels, offering increased convenience, speed, and performance over traditional handcast gels.
Advantages of the TGX™ and TGX Stain-Free™ FastCast™ acrylamide kits:
- Long shelf life — gels are good up to 1 month at 4°C after casting
- Accelerated workflow with 20 minute runs and 3 minute transfers
run handcast gels in 30 minutes
Additional benefits of TGX Stain-Free FastCast acrylamide kits:
- Visualize proteins in 5 minutes with stain-free technology
- No more fixing, staining, and destaining necessary
- Monitor success of electrophoresis and transfers through stain-free visualization of gels and membranes
Bio-Rad Launches New TGX™ FastCast™ and TGX Stain-Free™ FastCast™ Acrylamide Kits for Gel Electrophoresis
Bio-Rad Laboratories announced the launch of its TGX FastCast acrylamide and TGX Stain-Free FastCast acrylamide kits, which provide handcast gel users access to Bio-Rad’s TGX gel chemistry, previously available only in precast gels. The TGX FastCast kits allow users to hand cast polyacrylamide gels with fast run times, efficient protein transfers, and consistent, reproducible results. Researchers can also take advantage of the TGX Stain-Free version, which allows users to monitor the success of each step in their protein electrophoresis and western blotting workflows.
Fast Casting, Running, and Transfer Times, Long Shelf Life
Unlike traditional handcast gels, which require 60 minutes to polymerize before running, FastCast gels can be used only 30 minutes after casting, saving valuable preparation time. Protein electrophoresis can then be run on these gels in as little as 20 minutes, three times faster than conventional Tris-HCl handcast gels.
As with Bio-Rad’s TGX precast gels, proteins can be transferred from FastCast gels in as little as 3 minutes when used with Bio-Rad’s Trans-Blot® Turbo™ transfer system. In addition to these performance advantages, FastCast gels can conveniently be stored for up to 4 weeks after casting, allowing researchers to cast multiple gels at once and use as needed.
“Having a 4-week shelf life is extremely useful, as the handcast gels I previously worked with only lasted two to three days, forcing me to plan my experiments around this limited shelf life,” said Aaron Ramsey, a graduate student in the biochemistry department at Virginia Tech. “Now I can cast several gels at once that I know will last for several weeks.”
Benefits of Going Stain-Free
The TGX Stain-Free FastCast acrylamide kits allow protein detection in gels or on membranes in 3–5 minutes using Bio-Rad’s stain-free imaging systems. This makes it possible for researchers to cast their gels and achieve complete protein separation, gel imaging, and data analysis in less than an hour.
The importance of stain-free technology is readily apparent in western blotting experiments, where it provides confidence at each step of the workflow through visual checks. Stain-free visualization also enables total protein normalization, a more accurate method than using housekeeping proteins for correcting loading errors.
“The TGX Stain-Free FastCast acrylamide kits allow me to skip using extra antibodies for detecting loading controls because I can use a stain-free gel image for normalization to multiple proteins in the sample,” said Alan Cochrane, PhD, a professor in the Department of Molecular Genetics at the University of Toronto.
Dr. Cochrane also says that stain-free gels give him better sensitivity than Coomassie staining.
To learn more about the TGX FastCast and TGX Stain-Free FastCast acrylamide kits, visit www.bio-rad.com/FastCast-acrylamide.
Welcome to the sixth installment of the summer student tutorial series where summer students learn essential lab techniques without sapping the resources of their graduate student lab-mates. In this edition, you will learn how to make an SDS-PAGE Gel. Enjoy! And once again, if there are other tutorials you would be interested seeing in this space, please let us know and we will try to get it online as soon as possible.
50% off all Electrophoresis Buffers and Reagents – on-line only for a limited time.
Choose any of the following reagents and buffers:
- Acrylamide solutions
- Acrylamide powder
- Premixed Acrylamide/Bis Powders
- Premixed Gel-Casting Buffers
Premixed Buffers and Reagents:
- Protein Sample Loading Buffers
- Nucleic Acid Sample Loading Buffers
- Protein Running Buffers
- Nucleic Acid Running Buffers
- Blot Transfer and Processing Buffers
- Detergents and Blocking Reagents
- Tracking Dyes
- Bio-Lyte Ampholytes
- Cleaning Concentrate
- Gel Drying Solution
For more information visit the Bio-Rad Laboratories Canada Promotions Page.