Posts tagged ‘genomics’
For decades, a lucky subset of molecular biologists have been engaged in the study of beer diligently trying to figure out the science behind the drink’s clarity, taste and foam content. While most of us are content with studying beer after work hours, researchers from France and Japan worked many hours to uncover 18 proteins within the beer proteome that likely influence its delectable characteristics. Although their results are certainly noteworthy, the protocols utilized suffered from sample input limitations (less than 10 ml of sample per run) and consequently a relatively small number of proteins were discovered. Then came the Italians…
In a paper published in the Journal of Proteome Research , scientists from Milan utilized a protein enrichment strategy to identify 54 types of proteins from Italian-bottled Splügen beer. The group used Proteominer technology from Bio-Rad Laboratories to magnify low-abundant proteins that would have otherwise been lost with a protein depletion approach. They then analyzed the remaining fractions by mass spectrometry analysis and identified over 40 proteins that were present in trace amounts in the beer sample.
Lead author Pier Giorgio Righetti, of the The Polytechnic Institute of Milan told Discovery News “This opens up a completely new horizon in beer analysis in general, and also in the analysis of any beverage. We are now analyzing a lot of other beverages and finding a lot of surprising things that producers don’t know are in their beverages.”
Following hot on the heals of yesterdays post “A Practical Approach to Assay Design for qPCR“, we are proud to present you with another practical SlideShare on Fast qPCR assay optimization and validation techniques for HTS (high throughput screening). As with the previous presentation, the slide deck can be maximized for easier reading.
Designing good qPCR assays can be fun! Have a look at the presentation below to learn how to overcome difficult assays, designs and optimization while conforming to MIQE guidelines. If the slides are hard to read in their current format, click on the full screen button on the bottom right corner of the slide deck to enlarge.
An international research team led by the Research Institute of the McGill University Health Centre (RI MUHC) has made a major genetic breakthrough that could change the way pediatric cancers are treated in the future. The researchers identified two genetic mutations responsible for up to 40 per cent of glioblastomas in children – a fatal cancer of the brain that is unresponsive to chemo and radiotherapy treatment. The mutations were found to be involved in DNA regulation, which could explain the resistance to traditional treatments, and may have significant implications on the treatment of other cancers. The study was published this week in the journal Nature.
Below is a talk that was moderated this past summer by corresponding author Nada Jabado, Clinician and Principal Investigator at RI MUHC. The talk focuses on cancer initiation and cancer growth.
By far, one of the best sites on the web for learning about genomics is the NIH’s Talking Glossary of Genetic Terms. The website has a menu with a comprehensive list of relevant terms. Clicking on the hyperlinks gives you access to animated tutorials narrated by some of the best minds in the business along with non-copywrite images and 3D animations that help give viewers a clear understanding of the topic they are interested in.
For the experienced scientist, the website is a useful resource for helping explain to your friends and family exactly what you do in the lab and for finding illustrations to use in any way you choose (the site is in the public domain and therefore information may be freely distributed and copied).
I took the test and scored a 10/10 and I am attaching the certificate below to prove it. (I’m not going to tell you how many times I took the test before I received a perfect score;-)