Posts tagged ‘NGC chromatography system’
As you get ready to use your new NGC™ system, we will provide you with an opportunity to learn about the data evaluation tools built into the ChromLab software.
This training will cover chromatogram analysis of single run files, comparison of multiple run files, saving files, generating reports, and the column performance evaluation tool.
Have a Specialist contact me »
Bio-Rad Laboratories announced the launch of two new NGC Medium-Pressure Chromatography Systems, the NGC Discover™ and NGC™ Discover Pro Systems. The automation capabilities of both systems make them ideal for advanced purification applications such as method optimization and multi-step and tandem protein purification.
Bio-Rad optimized the Discover and Discover Pro Systems for pharmaceutical environments. These systems are available with a 10 ml/min pump module or with the recently released NGC 100 ml/min pump module, which enables flow rates up to 200 ml/min when used in combination with a mixer module. The Discover Systems further increase throughput by being able to load multiple samples and run up to 15 columns sequentially. In addition, the Discover Pro System can collect 12 large-volume fractions via its outlet valve. These higher flow rates and throughput capabilities — all under software control for maximum automation — will enable pharmaceutical researchers to maximize efficiency and productivity in the lab.
The Discover Systems’ best feature, however, may be their ease of use, a hallmark of the NGC family of chromatography instruments.
“Over the past 18 months, researchers have embraced NGC Systems due to their straightforward operation that allows even chromatography novices to get up to speed in only one or two runs,” said Kamni Vijay, Bio-Rad marketing director, Protein Function Division, Life Science Group. “By reimagining chromatography instrumentation from the ground up, we’ve created a system that’s an asset in making important scientific discoveries.”
All NGC Systems feature the same modular design with interchangeable plug-and-play modules that allow researchers to scale the system according to their throughput needs.
Other benefits of the NGC Chromatography System family include:
- Faster setup — the Point-to-Plumb™ feature offers step-by-step LED-guided plumbing of the system and quicker instrument setup
- Automated methods and analysis — ChromLab™ Software provides method templates that meet any purification need and it reduces tedious analysis with time-saving features such as single-click peak integration and the ability to stack and overlay chromatograms
- Robust performance and regulatory compliance — all systems can be operated in compliance with 21CFR Part 11 regulations using ChromLab Software Security Edition. System-level IQ/OQ validation and rigorous testing ensure that all systems live up to the high-throughput demands of a pharmaceutical environment
For more information about Bio-Rad’s suite of NGC Chromatography Systems, please visit www.bio-rad.com/NGCpr.
PPARg plays a role in glucose metabolism. Activated PPARg increases insulin sensitivity, making PPARg an important receptor for treatment of type 2 diabetes. Obtaining a crystal structure of PPARg aids in development of interacting ligands and beneficial anti-diabetic drugs. Here we describe the purification of PPARg using Bio-Rad’s NGCTM chromatography system and a three-step column purification approach. Well-ordered crystals of the purified PPARg protein were obtained. X-ray diffraction data showed consistent results compared to established protocols.
Structural biologist Petr Novák employs the latest techniques to model natural killer cell receptor molecules, research that may one day result in powerful therapies to treat cancer and infections. Go inside the fascinating world of structural characterization and find out how Novák has integrated the NGC™ chromatography system into his effort to visualize these molecules in vivid detail.
To talk about intuitive design, let’s start with something counterintuitive: When she began as the product manager for Bio-Rad’s effort to create an entirely new chromatography system, Farah Mavandadi had little experience with biochromatography.
“I’d used chromatography to perform small molecule purification, but never protein purification. It’s quite different,” she says. “I was actually intimidated by the BioLogic DuoFlow™ system [the preceding Bio-Rad chromatography system]. I used to bring an R&D scientist with me whenever I had to go for trainings on that system, just so I wouldn’t get lost.”
Far from being a liability, however, Mavandadi’s lack of experience in biochromatography would turn into a major advantage in designing an entirely new system.
New Bio-Rad promotional video. Thoughts?