Posts tagged ‘PCR’

The CFX Automation System II Has Arrived

Bio-Rad Laboratories announced the launch of its CFX Automation System II, a robotic plate handler that enables high-throughput, walk-away real-time PCR automation for all Bio-Rad CFX Real-Time PCR Detection Systems.

The new system is ideally suited to meet the high-throughput real-time PCR requirements of today’s drug discovery and screening workflows by facilitating hands-off, around-the-clock data generation and analysis.

The CFX Automation System II can be integrated with one or two CFX Systems while maintaining a small footprint, and includes easy-to-use software that makes automation accessible to all researchers. It is easily integrated with laboratory information management systems (LIMS) to facilitate sample tracking, save time, and minimize human error.

The CFX Automation System II allows laboratories to:

  • Save valuable lab space — operate two CFX Systems with a single plate handler using a fraction of the space required by other systems
  • Meet changing throughput demands — begin with one real-time system and scale up to two systems when required
  • Have confidence in your results — software allows for easy integration of wet-lab validated PrimePCR™ Assays (available in the U.S., Canada, Australia, New Zealand, Singapore, and E.U. only).

Please visit for more information about Bio-Rad’s CFX Automation II System.

November 4, 2014 at 3:39 pm Leave a comment

Ottawa Scientists Create (free) Program that Automates Primer Design

Bruno Fonseca, PhD scientist at the Children’s Hospital of Eastern Ontario Research Institute and Riu Liu, PhD scientist at the University of Southampton (United Kingdom) collaborated over the past four years to develop a software tool that automates the design of primers for site-directed mutagenesis. Their new software program is called PrimerGenesis.

“We’ve automated a Nobel-prize winning research technique,” says Bruno Fonseca, co-creator of the software. “Genome sequencing yields vast amounts of data on disease-causing mutations in DNA. Investigating each mutation by a common practice called site-directed mutagenesis is a laborious and time-consuming task. There’s an appetite for automating this task in the field of molecular biology research, so it’s been rewarding to develop this software.”

Generating mutations in a gene typically requires a researcher to manually design a small strip of DNA called “primer”. Instead of counting individual nucleotides within a strip of DNA, this time-consuming task can be completed computationally in a matter of seconds by using this new software program.

“We’ve aimed to make it as user-friendly as possible,” said Rui Liu, co-creator of PrimerGenesis. “The software is extremely versatile and can be widely applied; yes, it can be used to generate mutations but it can also be used to introduce specific tags upstream or downstream of a gene.”

PrimerGenesis is free and available globally without registration or download requirements at: or

Thanks to CHEO for contributing this story.

October 30, 2014 at 5:04 pm Leave a comment

PCR Perfect: Any Instrument, Any Condition, Any Time

To view the interactive tutorial visit

Bio-Rad PCR supermixes are designed with advanced enzyme and buffer formulations for robust amplification with even the most challenging templates and target sequences.

• Superior gene expression results — consistent performance in all cycling conditions
• Increased detection sensitivity and efficiency in compromised samples — Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors
• Decreased run times without compromising qPCR data quality
• Improved results with predeveloped qPCR assays — advanced formulation tolerates a broad range of reaction conditions, primer concentrations, and temperature ranges.

July 15, 2014 at 2:50 pm Leave a comment

Rethink What You Know About PCR

May 1, 2013 at 3:24 pm Leave a comment

Structural Biology Rap

April 25, 2013 at 10:28 am Leave a comment

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