Modernizing western blotting

Western blotting originated in the laboratory of George Stark at Stanford in 1979 (click here to read George’s autobiography published in JBC) and was later improved upon by other scientists around the world. While western blotting has become one of the most important techniques in the study of proteins, classical tank and semi-dry eletrophoretic transfer techniques have remained virtually unchanged for the past few decades.

Since the pace of scientific discovery grows exponentially over time it makes sense that classic techniques should be “modernized” to meet the challenges of our fast-paced research environment. Check out the video below to learn how Bio-Rad’s new Trans Blot Turbo helps researchers signinficantly speed up the pace of their protein transfer over classical tank and semi-dry blotting techniques.

Bio-Rad Video 5/11/11 from Lab Manager Magazine on Vimeo.

Keeping Proteins Soluble in 2D Gel Electrophoresis

2D Gel Electrophoresis is a staple for many proteomic biotechnology labs. In this Bio-Rad Proteomic Biotechnology video tutorial, you will learn what is needed to keep your proteins in solution for your 2D Gel Electrophoresis experiment. This video is the 5th part in a multi-series of proteomic workshops given by Dr. Ning Liu of Bio-Rad Laboratories. The videos were recorded live so the quality may not be perfect. If you are interested in having a proteomics workshop in your area, please email avi_wener@bio-rad.com.

Bio-Rad Tutorial: Protein Extraction for 2D Gel Electrophoresis

This is the fourth part in a series of proteomic workshops given by Dr. Ning Liu of Bio-Rad. The video series was captured from a live 2-day workshop on 2D electrophoresis and sample prep. In this clip you will learn the ideal ways to disrupt your cells in preparation for the IEF run.

2DGE Applications Tutorial

This is the third part in a series of proteomic workshops given by Dr. Ning Liu of Bio-Rad. The video series was captured from a live 2-day workshop on 2D electrophoresis and sample prep. In this clip you will learn about some relevant applications of 2D Gel Electrophoresis including protein profiling, differential display and post-translational modification.

How to generate a pH gradient for 2D Gel Electrophoresis

In this second part of our 2D Gel Electrophoresis workshop (see earlier post for introduction to 2DGE) Dr. Ning Liu describes how to generate a pH gradient and the use of IPG strips in modern 2DGE.

Introduction to 2D Gel Electrophoresis

A short while ago, Dr. Ning Liu of Bio-Rad Laboratories gave a 2-day hands on workshop on proteomic sample prep and 2D Gel Electrophoresis. Attendees learned the science behind 2DGE and what is required to get good results from this exteremly powerful proteomics technique (hint…sample prep is critical). Over the next few weeks we will be posting short clips from this workshop. The clips have been broken up into segments of under 10 minutes each so as not to overwhelm viewers with too much information in one sitting (it was a 2 day workshop afterall).

In this first clip you will be introduced to 2DGE and why it is such a powerful research tool.

Protein Extraction and Clean Up Tutorial

Below is part 2 in the Proteomics Workshop series. In this video you will learn the secrets to good sample preparation which is perhaps the most critical part to ANY proteomics experiment.