Coefficient of Variation Simplified

Simplifying one of stats most important calculations.

Prince Edward Island Biotechnology Job Openings

The Prince Edward Island Bioscience Cluster is the location of choice for companies engaged in the research, development, and commercialization of bioactive-based human and animal health and nutrition products. As part of our work in building the PEI Bioscience Cluster, the PEI BioAlliance is providing this section of our website to link professionals to careers in the PEI Bioscience sector by offering companies and organizations the opportunity to post available vacant positions.

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Canadian Activities at the BIO International Convention

One-stop resource for news and events happening this year for Canadians at the BIO International Convention in Boston from June 18th – 21st.

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Ask Questions: Evidence Based Management

Evidence based management (EBMgt) practitioners are taught to use multiple sources of data in their decision-making, including scientific evidence and research, in addition to relying on their own experience and the concerns of the people affected by their choices.

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Five common factors affecting protein transfer efficiency

If you are interested in obtaining the best protein transfer results, it is important to understand the relationship of the various factors involved. These factors include: voltage (V), current (I),resistance (R) and power (P).

Rapid protein transfers are generally obtained by applying maximum voltage while minimizing the distance betweeen electrodes. However, if the transfer conditions are inappropriate, poor transfer efficiency can result.

Two basic equations are important in electrophoresis.

The first is Ohm’s law: V = I x R

The second is the power equation: P = I x V = I2 x R = V2/R

While voltage and current are determined by the user and the power supply settings, the resistance is inherent in the system. It is therefore important to know which transfer conditions affect the resistance of the transfer system.

Below are the top five factors affecting resistance:

1. Alterations to buffer composition; that is, addition of SDS or changes in ion concentration due to addition of acid or base to adjust the pH of a buffer
2. Gel pH, ionic strength, and percentage of acrylamide, especially if the gel has not been properly equilibrated
3. Number of gels (current increases slightly as the number of gels increases)
4. Volume of buffer (current increases when volume increases)
5. Transfer temperature (current increases when temperature increases)

For more great information, be sure to download the Protein Blotting Guide from Bio-Rad Laboratories.