Posts tagged ‘biotechnology education’

Coefficient of Variation Simplified

Simplifying one of stats most important calculations.

July 24, 2012 at 11:04 am Leave a comment

Prince Edward Island Biotechnology Job Openings

The Prince Edward Island Bioscience Cluster is the location of choice for companies engaged in the research, development, and commercialization of bioactive-based human and animal health and nutrition products. As part of our work in building the PEI Bioscience Cluster, the PEI BioAlliance is providing this section of our website to link professionals to careers in the PEI Bioscience sector by offering companies and organizations the opportunity to post available vacant positions.

Click here for current postings.

June 20, 2012 at 7:30 pm Leave a comment

Canadian Activities at the BIO International Convention

One-stop resource for news and events happening this year for Canadians at the BIO International Convention in Boston from June 18th – 21st.

Click here for more

June 20, 2012 at 7:26 pm Leave a comment

Ask Questions: Evidence Based Management

Evidence based management (EBMgt) practitioners are taught to use multiple sources of data in their decision-making, including scientific evidence and research, in addition to relying on their own experience and the concerns of the people affected by their choices.

Click here for more.

June 20, 2012 at 7:18 pm Leave a comment

Five common factors affecting protein transfer efficiency

If you are interested in obtaining the best protein transfer results, it is important to understand the relationship of the various factors involved. These factors include: voltage (V), current (I),resistance (R) and power (P).

Rapid protein transfers are generally obtained by applying maximum voltage while minimizing the distance betweeen electrodes. However, if the transfer conditions are inappropriate, poor transfer efficiency can result.

Two basic equations are important in electrophoresis.

The first is Ohm’s law: V = I x R

The second is the power equation: P = I x V = I2 x R = V2/R

While voltage and current are determined by the user and the power supply settings, the resistance is inherent in the system. It is therefore important to know which transfer conditions affect the resistance of the transfer system.

Below are the top five factors affecting resistance:

  1. Alterations to buffer composition; that is, addition of SDS or changes in ion concentration due to addition of acid or base to adjust the pH of a buffer
  2. Gel pH, ionic strength, and percentage of acrylamide, especially if the gel has not been properly equilibrated
  3. Number of gels (current increases slightly as the number of gels increases)
  4. Volume of buffer (current increases when volume increases)
  5. Transfer temperature (current increases when temperature increases)

For more great information, be sure to download the Protein Blotting Guide from Bio-Rad Laboratories.

June 17, 2012 at 2:05 pm Leave a comment

Older Posts


Bio-Rad Canada has sponsored the development of this site to advance the productivity of the Canadian Biotechnology sector and the fine people who work in it across the country.  We invite readers to contribute content: posters, tools, research and presentations, articles white papers, multimedia, music downloads and entertainment, conference announcements, videos. Please contact for more information.

Bookmark and Share

Site developed by What If What Next(TM)

Follow us on Twitter

%d bloggers like this: