Posts tagged ‘western blotting’
Using Bio-Rad’s PDQuest™ Software to Generate a Match Rate between 2-D Electrophoresis and Western Blotting
Two-dimensional (2-D) electrophoresis is a widely used gel-based method for separating protein mixtures by charge (isoelectric point) and size (molecular weight). After 2-D electrophoresis (2-DE; described in detail in the Bio-Rad Applications & Technologies section), gels can be processed for protein detection via total protein staining and/or western blotting. Western blotting analysis following 2-DE separation is particularly useful in at least two important applications: (1) identification of pathogen-derived proteins that are highly immunogenic (have potential as vaccine candidates), and (2) evaluation of the range of host cell protein (HCP) impurities detectable by polyclonal antibodies used to monitor HCP impurities in purified biopharmaceuticals. For both applications, it is critical to generate a match rate from an overlay of the 2-DE and western blotting analyses. Bio-Rad’s PDQuest 2-D analysis software can be used to easily generate match rates. We describe here, step-by-step guidelines for generating this information using PDQuest software.
This week’s summer student tutorial is something that we have all experienced in the past. Leaky gels. While the best solution is probably to purchase a new pad, for the more resourceful scientists out there, here is a tip for you.
The video is courtesy of LabTricks which has no affiliation to Bio-Rad Laboratories.
Welcome to the third installment of the summer student tutorial series where summer students learn essential lab techniques without sapping the resources of their graduate student lab-mates. In this edition, you will learn how to set up and run a mini-PROTEAN TGX Precast Gel. Enjoy! And once again, if there are other tutorials you would be interested seeing in this space, please let us know and we will try to get it online as soon as possible.
We’re back with another edition of the Summer Tutorial Series. The series consists of helpful videos and tips and tricks that summer students, (and other lab folks requiring a refresher), that can be viewed at their leisure without being rushed by preoccupied lab techs and graduate students.
This week we present a mundane, yet essential, housekeeping tool that can make the difference between significant data and human error. Pipette Calibration is simple to do, yet neglected by many. Learn these lab essentials now, during your summer student days, and impress your much more experienced colleagues right from the get go.
Summer is here, and with summer comes the beloved summer student. While we all believe in the value of education, unfortunately, we may not have enough time on our hands dedicated to training these fresh recruits. That’s where The Canadian Biotechnologist can help! Over the next several weeks, we will post helpful videos and tips and tricks that summer students, (and other lab folks requiring a refresher), can view at their leisure without being rushed by preoccupied lab techs and graduate students. A true win-win for everyone involved.
This week we present the basics of Western Blotting. This is an essential technique for all molecular biologists and protein biochemists and the material in this video is very clear and easy to understand. If you find the video useful, please be sure to share it with your friends.
If there are other tutorials that you are interested in, please let us know and we will do our best to post it in this space as soon as possible.