Posts tagged ‘Cell Culture’
Commemorating the 60th Anniversary of Henrietta Lacks Death
Despite her tragic life as a poor black woman in America in the 1950’s (with all its associated implications), Henrietta Lacks was destined to become an invaluable source of progress to future scientific generations. A quick search of Pubmed reveals that over 68,000 papers have been published that reference HeLa cells with a number of Nobel Prize winning discoveries counted among them.
Tremendous credit should be given to Rebecca Skloot , author of “The Immortal Life of Henrietta Lacks,” for bringing Henrietta’s story to life and giving Henrietta and her family the recognition that they deserve.
This week marked the 60th anniversary of Henrietta’s death on October 4, 1951 at the tender age of 31. May she rest in peace and continue to be a source of pride for the scientific community and mankind.
October 5, 2011 at 10:39 am Canadian Biotechnologist Leave a comment
McGill Scientists invent a unique new tool for cell culture enthusiasts
A team from Montreal has invented a miniature cellular vacuum cleaner that can be used for selectively delivering molecular material to cells as well as creating chemical gradients used in experiments such as cell mobility studies.
The device is fabricated by etching four holes in a silicon tip, which is about 1 mm square. When the device is brought close to a surface, it acts on it pretty much like a water jet vacuum cleaner would. Two apertures (the “plus” holes, or sources) emit microscopic jets of fluid, onto the surface below and the two other apertures (the “minus” holes, or drains), immediately suck them back into the device.
In the vacuum cleaner analogy, if the carpet is replaced by a slice of living tissue, or a layer of adherent cells, the device can float over that surface to reach a desired target. It then simply sends out a stream of fluid with the chemicals needed to stimulate, probe, detach or kill the cells, depending on the application
Click here for further details.
September 28, 2011 at 7:27 am Canadian Biotechnologist Leave a comment
Fighting cancer with measels
Canadian researchers have discovered that a tumor cell marker is a receptor for measles virus, suggesting the possible use of measles virus to help fight cancer. Their findings appear in the Open Access journal PLoS Pathogens .
In a nutshell, Christopher D. Richardson and his team at Dalhousie University showed that measles virus can infect human airway epithelial cells and many adenocarcinoma cell lines. They also demonstrated that PVRL4, a tumour marker that is highly expressed on many lung, breast, colon, and ovarian tumors, converts cells that were resistant to measles viral infections, to cells that could support virus infections. Hence, the authors suggest that future experiments be done to investigate whether an oncolytic measels virus can be used to specifically target tumour cells expressing PVRL4, thus preferentially targetting lung, breast, colon and ovarian tumour cells.Click here for more.
Citation: Ryan S. Noyce, Daniel G. Bondre, Michael N. Ha, Liang-Tzung Lin, Gary Sisson, Ming-Sound Tsao, & Christopher D. Richardson (2011). Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus PLoS Pathogens : doi:10.1371/journal.ppat.1002240
September 7, 2011 at 8:31 am Canadian Biotechnologist Leave a comment
How to obtain statistically significant Western Blots
Western Blotting is probably one of the most ubiquitous techniques in the molecular biology lab and relatively easy to perform. Yet many of us have been frustrated with statistically insignificant results or protein bands that appear either too dark or too light to quantitate.
Well, do not despair! There are many things you can do to help improve the quality of your blots and increase your likelihood of obtaining statistically significant results!
In the video below, you will learn about the many factors affecting western blot analysis (such as detection limit and dynamic range limitations of film and overloaded gels) and what can be done to improve your chances of success.
The presentation was given by Bio-Rad Laboratories Field Application Specialist Dr. Sean Taylor as part of an intimate customer training. Some of the references in the presentation may be specific for that particular customer but the general information contained in this presentation is highly valuable to all molecular biology labs.
September 6, 2011 at 8:55 am Canadian Biotechnologist 1 comment
Cell counting as easy as 1,2,3
I hate counting cells. It is a tedious and boring process that is time consuming and inaccurate. However, there is another solution. Checkout this link with a video demonstration of Bio-Rad Laboratories TC-10 cell counter . Can you say Load, Insert and View Results? Can’t get much easier than that!
To schedule a live demo, fill out the contact info on the
September 5, 2011 at 9:24 am Canadian Biotechnologist Leave a comment